Genome editing by miniature CRISPR/Cas12f1 enzyme in Escherichia coli
نویسندگان
چکیده
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system is a valuable genome editing tool for microorganisms. However, the commonly used Cas9 nuclease derived from Streptococcus pyogenes (SpCas9) not applicable to many industrially relevant bacteria, due its cytotoxicity and large size (1368 amino acids [aa]). We developed an alternative using miniature Cas12f1 (529 aa) uncultured archaeon, Un1Cas12f1. When four dispensable genes in Escherichia coli MG1655 BW25113, CRISPR/Un1Cas12f1 showed higher efficiency (63%–100%) than CRISPR/SpCas9 (50%–79%). expected be applied of wide variety bacteria.
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ژورنال
عنوان ژورنال: Journal of Bioscience and Bioengineering
سال: 2021
ISSN: ['1347-4421', '1389-1723']
DOI: https://doi.org/10.1016/j.jbiosc.2021.04.009